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Microfluidic platform for culture and live cell imaging of cellular microarrays

Project Overview

Cellular microarrays contain populations of living cells that are spatially separated from one another. Due to the numerous discrete populations, these devices are beneficial in high-throughput screening applications. Last semester, we adapted a microfluidic device, originally created by Jeon et al., to be capable of establishing a concentration gradient over the intended cellular microarray portion of the platform. This semester, we aspire to integrate the microfluidic and microarray components in a way that enables them to be compatible with a standard microscope stage. Along with fitting into the stage, the platform must be able to generate concentration gradients across the field of flow, form a watertight seal, and be reusable in order for the device to be effective. By accomplishing this, our client will be able to perform live-cell imaging and high-throughput analysis to determine how various culture conditions affect neural stem cell differentiation.

Team Picture

Team members from left to right: Sarah Reichert (BWIG), John Byce (BSAC), Alex Johnson (Team Leader), Anthony Sprangers (Communicator)
Team members from left to right: Sarah Reichert (BWIG), John Byce (BSAC), Alex Johnson (Team Leader), Anthony Sprangers (Communicator)

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Contact Information

Team Members

  • Alexander Johnson - Team Leader
  • Anthony Sprangers - Communicator
  • John Byce - BSAC
  • Sarah Reichert - BWIG

Advisor and Client

  • Prof. John Puccinelli - Advisor
  • Prof. Randolph Ashton - Client

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