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Generation of an accessible and versatile hypoxia chamber

Project Overview

We are interested in understanding the impact of hypoxic stress on the behavior of cells transplanted to the heart following ischemic injury. One way to study this behavior is to attempt to mimic facets of the in vivo environment in an in vitro system. One facet we would like to control is the level of oxygen deprivation experienced by various cell types. Control of oxygen levels in cell culture has traditionally been achieved using large hypoxia chambers at one concentration at a time. Because of the large volume of air to be exchanged, hypoxia chambers are slow and cannot reach extreme levels of hypoxia. In addition, they are quite expensive to purchase. Microfluidic devices have been proposed to improve accessibility, versatility and so overall function of generated hypoxic environments. Significant progress has been made, but there is still substantial room for improvement. Thus, the goal of this design project will be to develop and validate a next-generation, microfluidic-based hypoxia chamber. The development of such a device would greatly benefit our studies as well as those of many other experimental investigations involving oxidative stress, ischemia, and reactive oxygen species (ROS)-mediated cellular pathways.

Team Picture

Denise, Roland, Sarvesh, Jason
Denise, Roland, Sarvesh, Jason

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Contact Information

Team Members

  • Roland Pomfret - Team Leader
  • Jiaquan Yu - Communicator
  • Lok Wong - BSAC
  • Sarvesh Periyasamy - BWIG

Advisor and Client

  • Prof. John Puccinelli - Advisor
  • Prof. Brenda Ogle - Client

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