CRISPRi screening in cancer spheroids to investigate factors in genome stability
Develop a cell culture method compatible with a 3D environment and CRISPRi screening in order to identify sources of DNA mutations in the tumor environment through γH2AX expression.
Project Overview
Although previous CRISPRi screening in 2D monolayers has provided useful knowledge on cancer drivers and therapeutic susceptibilities, it lacks an element of biological relevance to an in vivo environment. Therefore, our team was tasked with developing a cell culture method that is compatible with a 3D environment and CRISPRi screening in order to identify sources of DNA mutations in the tumor environment. On a high scale, the team must select a viable cell line for the screen, create and optimize a spheroid formation protocol, and develop a protocol to stain for γH2AX: a histone variant that acts as a sensitive marker for DNA damage.
Project Status
The team is finishing up the preliminary report, planning spheroid experiment 3, planning qPCR timeline, and will be completing Passage 12-15 this week.
Team Picture
Image
Files
- Preliminary Report (March 4, 2025)
- Preliminary Presentation (February 7, 2025)
Progress Reports
- Week 5 (February 27, 2025)
- Week 4 (February 20, 2025)
- Week 3 (February 13, 2025)
- Week 2 (February 6, 2025)
- Week 1 (January 30, 2025)
Contact Information
Team Members
- Althys Cao - Team Leader
- Ana Martinez - Communicator
- Emily Rhine - BSAC
- Julia Salita - BWIG
- Jayson O'Halloran - BPAG
Advisor and Client
- Prof. Paul Campagnola - Advisor
- Ms. Carley Schwartz - Client
- Dr. Gaelen Hess - Alternate Contact
Related Projects
- Spring 2025: CRISPRi screening in cancer spheroids to investigate factors in genome stability
- Fall 2024: CRISPRi screening in cancer spheroids to investigate factors in genome stability