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Microscope cell culture incubator

Develop a low cost cell culture incubation chamber with interchangeable culture plates that is compatible with an inverted microscope and capable of live cell imaging.

Project Overview

Develop a low cost cell culture incubation chamber with interchangeable culture plates that is compatible with an inverted microscope and capable of live cell imaging. This incubation chamber must be able to maintain an internal environment of 37 C, 5% CO2, and 95-100% humidity over a long duration of time, without compromising the integrity of the microscope's optics or functionality. Special consideration should be taken to maintain even heating and humidity across the chamber as gradients can result in evaporation from low volume cultures such as microfluidic devices. Current commercially available systems are prone to these issues and are extremely expensive. Commercial systems also tend to be large and enclose the entire microscope making it difficult to assemble and remove and between uses. Because of their size, they also hinder use of the microscope in general.

Team Picture

Top Row, Left to Right: Ethan Hannon (BSAC), Maya Tanna (Co-leader), Katie McGovern (Communicator). Bottom Row, Left to Right: Sam Bardwell (Co-leader), Olivia Jaekle (BWIG), Caroline Craig (BPAG)
Top Row, Left to Right: Ethan Hannon (BSAC), Maya Tanna (Co-leader), Katie McGovern (Communicator). Bottom Row, Left to Right: Sam Bardwell (Co-leader), Olivia Jaekle (BWIG), Caroline Craig (BPAG)

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Contact Information

Team Members

  • Maya Tanna - Co-Team Leader
  • Samuel Bardwell - Co-Team Leader
  • Katie Day - Communicator
  • Ethan Hannon - BSAC
  • Olivia Jaekle - BWIG
  • Caroline Craig - BPAG

Advisor and Client

  • Dr. Melissa Kinney - Advisor
  • Prof. John Puccinelli - Client

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