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Microscope cell culture incubator

The goal of this project is to create a cell culture incubator system that can be used in the BME teaching labs for instructional purposes.

Project Overview

Develop a low cost cell culture incubation chamber that is compatible with an inverted microscope and capable of live cell imaging. This incubation chamber must be able to maintain an internal environment of 37 C, 5% CO2, and 95-100% humidity over a long duration of time, without compromising the integrity of the microscope's optics or functionality. Special consideration should be taken to maintain even heating and humidity across the chamber as gradients can result in evaporation from low volume cultures such as microfluidic devices. Current commercially available systems are prone to these issues and are extremely expensive. Commercial systems also tend to be large and enclose the entire microscope making it difficult to assemble and remove and between uses. Because of their size, they also hinder use of the microscope in general.

Team Picture

Top Row, Left to Right: Bella Raykowski (BSAC), Katie Day (Communicator). Bottom Row, Left to Right: Sam Bardwell (Leader), Drew Hardwick (BPAG), Maya Tanna (BWIG)
Top Row, Left to Right: Bella Raykowski (BSAC), Katie Day (Communicator). Bottom Row, Left to Right: Sam Bardwell (Leader), Drew Hardwick (BPAG), Maya Tanna (BWIG)

Files

Contact Information

Team Members

  • Samuel Bardwell - Team Leader
  • Katie Day - Communicator
  • Bella Raykowski - BSAC
  • Maya Tanna - BWIG
  • Drew Hardwick - BPAG

Advisor and Client

  • Dr. Melissa Kinney - Advisor
  • Prof. John Puccinelli - Client

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